Saito T, Yamaguchi I
Department of Animal Science, Faculty of Agriculture, Kobe University, Japan.
J Vet Med Sci. 2000 Jun;62(6):575-81. doi: 10.1292/jvms.62.575.
N-glycosylation and glucose trimming of the influenza virus hemagglutinin (HA) and neuraminidase (NA) were studied by using glycosylation inhibitor (tunicamycin; TM) and glucosidase inhibitors. TM treatment of MDCK cells infected with a reassortant virus NWS-N8 resulted in reduced transport of the viral glycoproteins to the cell surface. The degree of the effects differed between the HA and the NA (80% reduction for the HA and 97% reduction for the NA), indicating a difference in dependency on N-glycosylation between these glycoproteins. Differential dependency on glucose trimming was clearly demonstrated when the surface transport of the glycoproteins was compared after treatment of the virus-infected cells with glucosidase inhibitors. Fluorescence-activated cell sorting (FACS) analysis revealed that the surface transport of the NA reduced to 50% after castanospermine (CST) treatment but not did that of the HA. An anti-viral effect of a glucosidase inhibitor on the NWS-N8 strain was also demonstrated. The correlation between the expression of the NA on the cell surface and virus yield suggests that CST may interfere with virus release through its effect on the NA.
利用糖基化抑制剂（衣霉素；TM）和葡萄糖苷酶抑制剂，对流感病毒血凝素（HA）和神经氨酸酶（NA）的N-糖基化及葡萄糖修剪进行了研究。用TM处理感染重配病毒NWS-N8的MDCK细胞，导致病毒糖蛋白向细胞表面的转运减少。HA和NA的影响程度有所不同（HA减少80%，NA减少97%），表明这些糖蛋白对N-糖基化的依赖性存在差异。在用葡萄糖苷酶抑制剂处理病毒感染细胞后，比较糖蛋白的表面转运时，清楚地证明了对葡萄糖修剪的不同依赖性。荧光激活细胞分选（FACS）分析显示，粟精胺（CST）处理后，NA的表面转运减少到50%，而HA没有。还证明了葡萄糖苷酶抑制剂对NWS-N8毒株的抗病毒作用。细胞表面NA的表达与病毒产量之间的相关性表明，CST可能通过其对NA的作用干扰病毒释放。
