Kuan K N, Lee Y Y, Melius P
Biochem J. 1979 Mar 1;177(3):981-3. doi: 10.1042/bj1770981.
An improved procedure was developed whereby a primary light signal can be intensified and made visible by activation of a pre-tyrosinase (pre-phenoloxidase) enzyme [isolated from silkworm (Bombyx mori)] by alpha-chymotrypsin; this activation results from the light-activated conversion of the inactive cis-cinnamoyl-alpha-chymotrypsin.
开发了一种改进的方法,通过α-胰凝乳蛋白酶激活一种前酪氨酸酶(前酚氧化酶)[从家蚕(Bombyx mori)中分离得到],从而增强初级光信号并使其可见;这种激活是由无活性的顺式肉桂酰-α-胰凝乳蛋白酶的光激活转化产生的。