Nowak J Z, Kuba K, Zawilska J B
Department of Biogenic Amines, Polish Academy of Sciences, Lódź, Poland.
Acta Neurobiol Exp (Wars). 2000;60(2):209-14. doi: 10.55782/ane-2000-1339.
Two molecular forms of pituitary adenylate cyclase-activating polypeptide (PACAP), i.e., PACAP27 and PACAP38 (0.0001-1 microM), as well as vasoactive intestinal polypeptide (VIP; 0.1-3 microM), have been studied for their effects on cyclic AMP formation in the hypothalamus and cerebral cortex of duck and goose. All three peptides concentration-dependently stimulated cyclic AMP production in the tested brain regions of 2-3-weeks-old (young) ducks, with VIP showing at least one order of magnitude weaker activity than PACAP. This characteristics suggests the existence in the duck's brain of adenylyl cyclase-linked PAC1 receptors. Both forms of PACAP also stimulated the nucleotide formation in the cerebral cortex and hypothalamus of 5-6-months-old (adult) ducks or geese grown under natural environment. The peptides-evoked effects in adult and young ducks were comparable, and clearly greater than those found in adult geese. The present data extend our recent observations made on chicks, and suggest PACAP to be a potent stimulator of the cyclic AMP generation in the avian central nervous system.
研究了垂体腺苷酸环化酶激活多肽（PACAP）的两种分子形式，即PACAP27和PACAP38（0.0001 - 1微摩尔），以及血管活性肠肽（VIP；0.1 - 3微摩尔）对鸭和鹅下丘脑及大脑皮层中环磷酸腺苷（cAMP）生成的影响。在2至3周龄（幼龄）鸭的受试脑区，所有这三种肽均呈浓度依赖性地刺激cAMP生成，其中VIP的活性比PACAP至少弱一个数量级。这一特性表明鸭脑中存在与腺苷酸环化酶相连的PAC1受体。两种形式的PACAP还刺激了自然环境下生长的5至6月龄（成年）鸭或鹅的大脑皮层和下丘脑中核苷酸的生成。在成年鸭和幼龄鸭中，这些肽引发的效应相当，且明显大于成年鹅中的效应。目前的数据扩展了我们最近对雏鸡的观察结果，并表明PACAP是禽类中枢神经系统中cAMP生成的有效刺激物。
