DePhillips P, Lenhoff A M
Merck Research Laboratories, West Point, PA 19486, USA.
J Chromatogr A. 2000 Jun 23;883(1-2):39-54. doi: 10.1016/s0021-9673(00)00420-9.
The pore dimensions, pore size distributions, and phase ratios were determined for a set of cation-exchange adsorbents using inverse size-exclusion chromatography (ISEC). The adsorbents examined represent a diverse set of materials from Pharmacia, TosoHaas, BioSepra, and EM Industries, which are widely used for protein purification. The ISEC was carried out using dextran standards with relative molecular masses of 180-6,105,000. This technique provided a comparative characterization of the accessible internal pore surface area, as a function of solute size, for the adsorbents tested. Adsorbent preparation strategies in which polymers are generated in situ or grafted onto base materials were found to have significant effects on pore dimensions and phase ratios.
使用反相尺寸排阻色谱法（ISEC）测定了一组阳离子交换吸附剂的孔径尺寸、孔径分布和相比。所研究的吸附剂代表了来自Pharmacia、TosoHaas、BioSepra和EM Industries的多种材料，这些材料广泛用于蛋白质纯化。ISEC使用相对分子质量为180 - 6,105,000的葡聚糖标准品进行。该技术提供了所测试吸附剂可及内部孔表面积随溶质尺寸变化的比较表征。发现聚合物原位生成或接枝到基材上的吸附剂制备策略对孔径尺寸和相比有显著影响。
