Epperson A, Hatton W J, Callaghan B, Doherty P, Walker R L, Sanders K M, Ward S M, Horowitz B
Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno 89557, USA.
Am J Physiol Cell Physiol. 2000 Aug;279(2):C529-39. doi: 10.1152/ajpcell.2000.279.2.C529.
Located within the tunica muscularis of the gastrointestinal (GI) tract are networks of cells known as interstitial cells of Cajal (ICC). ICC are critical for important basic functions of GI motility such as generation and propagation of slow-wave pacemaker activity and reception of regulatory inputs from the enteric nervous system. We have developed a novel procedure to identify and isolate individual ICC from freshly dispersed cell preparations of the murine small intestine and gastric fundus and to determine differential transcriptional expression We have compared the expression profiles of pacemaker ICC isolated from the murine small intestine (IC-MY) and ICC involved in neurotransmission from the gastric fundus (IC-IM). We have also compared expression profiles between ICC and smooth muscle cells (SMC) and between freshly isolated ICC and cultured ICC. Cultured ICC express smooth muscle myosin, whereas freshly dispersed ICC do not. All cell types express muscarinic receptor types M(2) and M(3), neurokinin receptors NK(1) and NK(3), and inhibitory receptor VIP-1, whereas only cultured ICC and SMC express VIP-2. Both cultured and freshly dispersed IC-IM and IC-MY express the soluble form of stem cell factor, whereas SMC from the gastric fundus express only the membrane-bound form.
位于胃肠道（GI）肌层内的是 Cajal 间质细胞（ICC）网络。ICC 对于胃肠道运动的重要基本功能至关重要，例如慢波起搏器活动的产生和传播以及来自肠神经系统的调节输入的接收。我们开发了一种新方法，可从新鲜分散的小鼠小肠和胃底细胞制剂中识别和分离单个 ICC，并确定差异转录表达。我们比较了从小鼠小肠分离的起搏器 ICC（IC-MY）和参与胃底神经传递的 ICC（IC-IM）的表达谱。我们还比较了 ICC 和平滑肌细胞（SMC）之间以及新鲜分离的 ICC 和培养的 ICC 之间的表达谱。培养的 ICC 表达平滑肌肌球蛋白，而新鲜分散的 ICC 则不表达。所有细胞类型均表达毒蕈碱受体 M(2)和 M(3)、神经激肽受体 NK(1)和 NK(3)以及抑制性受体 VIP-1，而只有培养的 ICC 和 SMC 表达 VIP-2。培养的和新鲜分散的 IC-IM 和 IC-MY 均表达可溶性干细胞因子形式，而胃底的 SMC 仅表达膜结合形式。
