Rippe C, Berger K, Böiers C, Ricquier D, Erlanson-Albertsson C
Section for Molecular Signaling, Department of Cell and Molecular Biology, University of Lund, S-221 00 Lund, Sweden.
Am J Physiol Endocrinol Metab. 2000 Aug;279(2):E293-300. doi: 10.1152/ajpendo.2000.279.2.E293.
Nonshivering thermogenesis induced in brown adipose tissue (BAT) during high-fat feeding is mediated through uncoupling protein 1 (UCP1). UCP2 is a recently identified homologue found in many tissues. To determine the role of UCP1 and UCP2 in thermoregulation and energy balance, we investigated the long-term effect of high-fat feeding on mRNA levels in mice at two different ambient temperatures. We also treated mice with the anorectic peptide enterostatin and compared mRNA levels in BAT, white adipose tissue (WAT), stomach, and duodenum. Here, we report that high-fat feeding at 23 degrees C increased UCP1 and UCP2 levels in BAT four- and threefold, respectively, and increased UCP2 levels fourfold in WAT. However, at 29 degrees C, UCP1 decreased, whereas UCP2 remained unchanged in BAT and increased twofold in WAT. Enterostatin increased UCP1 and decreased UCP2 mRNA in BAT. In stomach and duodenum, high-fat feeding decreased UCP2 mRNA, whereas enterostatin increased it. Our results suggest that the regulation of uncoupling protein mRNA levels by high-fat feeding is dependent on ambient temperature and that enterostatin is able to modulate it.
高脂喂养期间棕色脂肪组织（BAT）中诱导产生的非颤抖性产热是通过解偶联蛋白1（UCP1）介导的。UCP2是最近在许多组织中发现的同源物。为了确定UCP1和UCP2在体温调节和能量平衡中的作用，我们研究了高脂喂养在两种不同环境温度下对小鼠mRNA水平的长期影响。我们还用厌食肽肠抑胃肽处理小鼠，并比较了BAT、白色脂肪组织（WAT）、胃和十二指肠中的mRNA水平。在此，我们报告，在23摄氏度下高脂喂养分别使BAT中的UCP1和UCP2水平增加了四倍和三倍，并使WAT中的UCP2水平增加了四倍。然而，在29摄氏度时，BAT中的UCP1减少，而UCP2保持不变，WAT中的UCP2增加了两倍。肠抑胃肽增加了BAT中的UCP1并降低了UCP2 mRNA水平。在胃和十二指肠中，高脂喂养降低了UCP2 mRNA水平，而肠抑胃肽则使其增加。我们的结果表明，高脂喂养对解偶联蛋白mRNA水平的调节取决于环境温度，并且肠抑胃肽能够对其进行调节。
