Arndt M, Lendeckel U, Spiess A, Faust J, Neubert K, Reinhold D, Ansorge S
Institute of Immunology, Centre of Internal Medicine, Otto von Guericke University, Leipziger Strasse 44, Magdeburg, D-39120,
Biochem Biophys Res Commun. 2000 Aug 2;274(2):410-4. doi: 10.1006/bbrc.2000.3144.
Stimulation of human T-cells by pokeweed mitogen (PWM) results in a significant increase of IL-2, IFN-gamma, and DP IV mRNA expression as analyzed by quantitative RT-PCR. Here we show for the first time that the changes observed in cytokine mRNA expression are dose-dependently suppressed by the specific dipeptidyl peptidase IV inhibitor Lys[Z(NO(2))]-thiazolidide. Most interestingly, the inhibition of DP IV activity leads to a decrease in mRNA expression of the enzyme itself. Furthermore, evidence is provided that this suppression is mediated by TGF-beta(1). The presented data fit into the hypothesis that inhibition of DP IV leads to the induction of TGF-beta(1), which in turn provokes an arrest of cell cycle in late G(1).
