Ishii K, Araie M
Eye Clinic, Omiya Red Cross Hospital, Yono, Saitama, Japan.
Jpn J Ophthalmol. 2000 May-Jun;44(3):227-34. doi: 10.1016/s0021-5155(99)00217-8.
To evaluate the effects of topical latanoprost and timolol in combined therapy on retinal blood flow and tissue circulation in the optic nerve head (ONH) of the cynomolgus monkey.
Latanoprost (30 microL, 0.005%) was instilled once daily and timolol (30 microL, 0.5%) twice daily for 6 days into 1 eye, and physiological saline into the other eye to serve as control. Blood velocity through retinal veins was determined using Kowa Laser Speckle Blood Flow Meter. The ONH tissue blood velocity (NB(ONH)) was determined using the Laser Speckle Tissue Circulation Analyzer. Retinal blood flow and NB(ONH) determinations were carried out before the first instillation and 4 hours after the first instillation on the 2nd experimental day, and the last instillation at the same sites at the same time of day. The intraocular pressure (IOP) was also measured periodically.
After the first instillation, on the 2nd experimental day, the retinal blood flow decreased compared with the baseline, but 6-day instillation caused no significant change from the baseline. Six-day instillation increased the NB(ONH) in the treated eyes by 10% from the baseline and by 7% from that in the fellow control eye. After 6-day instillation, the IOP was lowered by 7.8+/-2.7 mm Hg and 3.6+/-4.3 mm Hg in the treated and control eyes, respectively.
Combined 6-day instillation of latanoprost once daily and timolol twice daily has no significant effect on the retinal blood flow, but significantly increases the ONH tissue blood velocity in monkey eyes. Jpn J Opthalmol 2000;44:227-234
评估局部用拉坦前列素和噻吗洛尔联合治疗对食蟹猴视神经乳头（ONH）视网膜血流和组织循环的影响。
将拉坦前列素（30微升，0.005%）每日滴注1次，噻吗洛尔（30微升，0.5%）每日滴注2次，持续6天，滴入1只眼睛，另一只眼睛滴入生理盐水作为对照。使用Kowa激光散斑血流仪测定视网膜静脉的血流速度。使用激光散斑组织循环分析仪测定ONH组织血流速度（NB(ONH)）。在第2个实验日首次滴注前以及首次滴注后4小时，在同一天的同一部位进行末次滴注时，测定视网膜血流和NB(ONH)。还定期测量眼压（IOP）。
首次滴注后，在第2个实验日，视网膜血流较基线下降，但6天滴注后与基线相比无显著变化。6天滴注使治疗眼的NB(ONH)较基线增加了10%，较对侧对照眼增加了7%。6天滴注后，治疗眼和对照眼的眼压分别降低了7.8±2.7毫米汞柱和3.6±4.3毫米汞柱。
每日1次拉坦前列素和每日2次噻吗洛尔联合滴注6天对视网膜血流无显著影响，但可显著增加猴眼的ONH组织血流速度。《日本眼科杂志》2000年；44:227 - 234
