Zeppa S, Vallorani L, Potenza L, Bernardini F, Pieretti B, Guescini M, Giomaro G, Stocchi V
Istituto di Chimica 'Giorgio Fornaini' Università degli Studi di Urbino, 61029 Urbino, Italy.
FEMS Microbiol Lett. 2000 Jul 15;188(2):119-24. doi: 10.1111/j.1574-6968.2000.tb09181.x.
Very little information is available to date about the complex truffle life cycle which involves the succession of three developmental phases. In order to gain more knowledge about ectomycorrhizal formation and fruit body development an ectomycorrhizal model system was used to study fungal biomass and plant and fungal transcript levels. They were evaluated in ectomycorrhizal development using the ergosterol assay and the internal transcribed spacer-5.8S ribosomal DNA from Tilia platyphyllos and Tuber borchii as molecular probes respectively. The results obtained from different approaches revealed a decrease in fungal biomass, transcript and protein levels during ectomycorrhizal development.
迄今为止，关于复杂的块菌生命周期的信息非常少，其生命周期涉及三个发育阶段的连续过程。为了获得更多关于外生菌根形成和子实体发育的知识，使用了一个外生菌根模型系统来研究真菌生物量以及植物和真菌的转录水平。在菌根发育过程中，分别使用麦角固醇测定法以及来自阔叶椴和夏块菌的内转录间隔区-5.8S核糖体DNA作为分子探针来对它们进行评估。从不同方法获得的结果显示，在外生菌根发育过程中真菌生物量、转录物和蛋白质水平均有所下降。
