Chang S Y, Vithayasai V, Vithayasai P, Essex M, Lee T H
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
J Infect Dis. 2000 Aug;182(2):442-50. doi: 10.1086/315730. Epub 2000 Jul 21.
A series of recombinant peptides of the human immunodeficiency virus type 1 (HIV-1) subtype E envelope were used to address the question of whether immunogenic epitopes similar to those described for the subtype B envelope are also present in structurally analogous regions of another HIV-1 subtype with divergent sequences. Five recombinant peptides, covering the V2 and V3 domains of gp120, the cysteine-loop region of gp41, a gp41 region involved in oligomerization, and the cytoplasmic tail of gp41, were found to react with >50% of the serum samples analyzed. All but the V2 region in the HIV-1 subtype B envelope have been reported to contain continuous epitopes that are highly immunogenic during natural infection. This finding suggests that, despite the sequence divergence between subtype E and B envelopes, most of the continuous epitopes that are highly immunogenic during natural infection are located at structurally analogous regions of the envelope.
使用一系列1型人类免疫缺陷病毒（HIV-1）E亚型包膜重组肽，来探讨在序列不同的另一种HIV-1亚型的结构类似区域中，是否也存在与B亚型包膜中所描述的那些具有免疫原性的表位。发现覆盖gp120的V2和V3结构域、gp41的半胱氨酸环区域、参与寡聚化的gp41区域以及gp41细胞质尾部的五种重组肽，与所分析的超过50%的血清样本发生反应。据报道，除了HIV-1 B亚型包膜中的V2区域外，所有这些区域都含有在自然感染期间具有高度免疫原性的连续表位。这一发现表明，尽管E亚型和B亚型包膜之间存在序列差异，但在自然感染期间具有高度免疫原性的大多数连续表位都位于包膜的结构类似区域。
