粟酒裂殖酵母α-半乳糖苷酶MEL基因的核苷酸序列。

Nucleotide sequence of alpha-galactosidase MEL gene from Zygosaccharomyces mrakii.

作者信息

Oda Y, Fujisawa T

机构信息

Upland Agriculture Research Center, Hokkaido National Agricultural Experiment Station, Memuro, Kasai, Hokkaido, 082-0071, Japan.

出版信息

Curr Microbiol. 2000 Sep;41(3):220-2. doi: 10.1007/s002840010123.

DOI:10.1007/s002840010123

PMID:10915212

Abstract

The region encompassing the alpha-galactosidase MELr gene was amplified from Zygosaccharomyces mrakii IFO 1835(T) by inverse-PCR and then sequenced. The nucleotide sequence of this region revealed a single open reading frame of 1410 bp encoding a 470 amino acid protein with a molecular weight of 51,909. The similarity of the deduced mature protein to other yeast alpha-galactosidases was 63.3% to Zygosaccharomyces cidri, 71.5% to Torulaspora delbrueckii, and 70. 7-73.9% to Saccharomyces species. The nucleotide and amino acid sequences are deposited in the DDBJ/EMBL/GenBank database under Accession Number AB030209.

摘要

通过反向PCR从粟酒裂殖酵母IFO 1835(T)中扩增出包含α-半乳糖苷酶MELr基因的区域，然后进行测序。该区域的核苷酸序列显示有一个1410 bp的单一开放阅读框，编码一个470个氨基酸的蛋白质，分子量为51,909。推导的成熟蛋白与其他酵母α-半乳糖苷酶的相似性为：与柠檬形接合酵母的相似性为63.3%，与德巴利酵母的相似性为71.5%，与酿酒酵母属的相似性为70.7 - 73.9%。核苷酸和氨基酸序列已保存在DDBJ/EMBL/GenBank数据库中，登录号为AB030209。