在携带去阻遏 F 样 R 因子的大肠杆菌细胞外膜中检测到一种类似于性菌毛亚基的蛋白质。
Detection of a protein, similar to the sex pilus subunit, in the outer membrane of Escherichia coli cells carrying a derepressed F-like R factor.
作者信息
Beard J P, Connolly J C
出版信息
J Bacteriol. 1975 Apr;122(1):59-65. doi: 10.1128/jb.122.1.59-65.1975.
Abstract
The outer membranes of Escherichia coli K-12 cells carrying a derepressed F-like R factor contain about 7 times 10-4 molecules per cell of a protein similar to the subunit of the sex pili specified by the R factor. This protein pool is absent in cells carrying the repressed variant of the R factor. The size of the pool is about one-half of the amount of protein incorporated into mature sex pili at the peak of production and is independent of the phase of growth of the culture. The molecular weight of the protein in the pool and of the subunit of the sex pili specified by the cells is 12,500 plus or minus 600.
摘要
携带去阻遏 F 样 R 因子的大肠杆菌 K-12 细胞的外膜，每个细胞含有约 7×10⁻⁴个分子的一种蛋白质，该蛋白质类似于 R 因子所规定的性菌毛亚基。携带 R 因子阻遏变体的细胞中不存在这种蛋白质库。该库的大小约为成熟性菌毛产量峰值时掺入蛋白质总量的一半，且与培养物的生长阶段无关。库中蛋白质和细胞所规定的性菌毛亚基的分子量为 12500±600。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
3
A micro biuret method for protein determination; determination of total protein in cerebrospinal fluid.
Scand J Clin Lab Invest. 1953;5(3):218-22. doi: 10.3109/00365515309094189.
4
Mixed infections with bacterial sex factors: sex pili of pure and mixed phenotype.
Ann Inst Pasteur (Paris). 1971 Jan;120(1):3-8.
5
The molecular weight of the undegraded polypeptide chain of yeast hexokinase.
Biochem Biophys Res Commun. 1970 Apr 8;39(1):46-52. doi: 10.1016/0006-291x(70)90755-2.
6
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
7
The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria.
Trans N Y Acad Sci. 1965 Jun;27(8):1003-54. doi: 10.1111/j.2164-0947.1965.tb02342.x.
8
Functions of F pili in mating-pair formation and male bacteriophage infection studies by blending spectra and reappearance kinetics.
J Bacteriol. 1969 Jun;98(3):1307-19. doi: 10.1128/jb.98.3.1307-1319.1969.
9
Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids.
Bacteriol Rev. 1968 Mar;32(1):55-83. doi: 10.1128/br.32.1.55-83.1968.
10
Structure and function of the cell envelope of gram-negative bacteria.
Bacteriol Rev. 1974 Mar;38(1):87-110. doi: 10.1128/br.38.1.87-110.1974.
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