Con突变体：大肠杆菌K-12中的一类突变体，缺乏一种主要的细胞壁蛋白，在噬菌体的接合和吸附方面存在缺陷。
Con--mutants: class of mutants in Escherichia coli K-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage.
作者信息
Skurray R A, Hancock R E, Reeves P
出版信息
J Bacteriol. 1974 Sep;119(3):726-35. doi: 10.1128/jb.119.3.726-735.1974.
Abstract
We describe a new class of mutants of Escherichia coli K-12 defective in conjugation (Con(-) mutants). They lack a major protein of the outer membrane and are defective as recipients with Hfr and F' donors and as recipients for several F-like R factors and Col factors. In the case of crosses with an Hfr donor, we have shown that the Con(-) recipient is defective in pair formation. The mutants are resistant to certain phages due to loss of receptor activity.
摘要
我们描述了一类新的大肠杆菌K-12接合缺陷型突变体（Con(-)突变体）。它们缺乏外膜的一种主要蛋白质，作为Hfr和F'供体的受体以及几种F样R因子和Col因子的受体存在缺陷。在与Hfr供体杂交的情况下，我们已经表明Con(-)受体在配对形成方面存在缺陷。由于受体活性丧失，这些突变体对某些噬菌体具有抗性。
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本文引用的文献
1
IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI.改进的大肠杆菌胸腺嘧啶需求突变体分离方法。
J Bacteriol. 1965 Aug;90(2):554-5. doi: 10.1128/jb.90.2.554-555.1965.
2
ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12.大肠杆菌K12重组缺陷突变体的分离与鉴定
Proc Natl Acad Sci U S A. 1965 Feb;53(2):451-9. doi: 10.1073/pnas.53.2.451.
3
A multiple-syringe bacteriophage applicator.一种多注射器噬菌体涂抹器。
Am J Clin Pathol. 1960 Mar;33:233-7. doi: 10.1093/ajcp/33.3.233.
4
A proposal for a uniform nomenclature in bacterial genetics.细菌遗传学统一命名法的提议。
Genetics. 1966 Jul;54(1):61-76. doi: 10.1093/genetics/54.1.61.
5
Acrylamide-gel electrophorograms by mechanical fractionation: radioactive adenovirus proteins.通过机械分级分离得到的丙烯酰胺凝胶电泳图谱：放射性腺病毒蛋白
Science. 1966 Feb 25;151(3713):988-90. doi: 10.1126/science.151.3713.988.
6
A new method for the extraction of R lipopolysaccharides.一种提取R脂多糖的新方法。
Eur J Biochem. 1969 Jun;9(2):245-9. doi: 10.1111/j.1432-1033.1969.tb00601.x.
7
Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.在不连续缓冲系统中通过凝胶电泳测定蛋白质 - 十二烷基硫酸盐复合物的分子量
J Biol Chem. 1971 Oct 25;246(20):6328-34.
8
Toward a metabolic interpretation of genetic recombination of E. coli and its phages.关于大肠杆菌及其噬菌体基因重组的代谢解读
Annu Rev Microbiol. 1971;25:437-64. doi: 10.1146/annurev.mi.25.100171.002253.
9
Resistance of Escherichia coli to penicillins. IX. Genetics and physiology of class II ampicillin-resistant mutants that are galactose negative or sensitive to bacteriophage C21, or both.大肠杆菌对青霉素的抗性。IX. 对噬菌体C21呈半乳糖阴性或敏感或两者兼具的II类氨苄青霉素抗性突变体的遗传学和生理学特性
J Bacteriol. 1971 Dec;108(3):1210-23. doi: 10.1128/jb.108.3.1210-1223.1971.
10
Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100.用曲拉通X-100溶解大肠杆菌的细胞质膜。
J Bacteriol. 1971 Oct;108(1):545-52. doi: 10.1128/jb.108.1.545-552.1971.
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