Chien T I, Chen Y G, Chiang B L
Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei, ROC.
J Microbiol Immunol Infect. 2000 Jun;33(2):87-92.
It has been well documented that the mite Dermatophoides pteronyssinus (Der p) is the major allergen in house dust in Taiwan. The purpose of this study is to develop a sandwich immunometric assay which can be used for the standardization of mite allergen extracts or house dust, for assessing the procedures of mite avoidance, and for identifying species in epidemiological studies. Der p 1 allergen (both recombinant and native Der p 1) was purified and a panel of Der p 1 specific monoclonal antibodies was developed. The monoclonal antibodies were biotinylated and a two-site enzyme-linked immunosorbent assay (ELISA) with peroxidase-conjugated streptavidin was developed for the detection of Der p 1 allergen. The results suggest that these monoclonal antibodies could be applied both in affinity columns for the purification of native Der p 1 antigen and in sandwich-ELISA for epidemiological study.
已有充分文献记载，粉尘螨是台湾室内灰尘中的主要过敏原。本研究的目的是开发一种夹心免疫测定法，可用于尘螨过敏原提取物或室内灰尘的标准化，评估避免接触尘螨的措施，并在流行病学研究中识别物种。纯化了重组和天然的粉尘螨变应原Der p 1，并制备了一组Der p 1特异性单克隆抗体。将单克隆抗体进行生物素化，并开发了一种用辣根过氧化物酶标记的链霉亲和素的双位点酶联免疫吸附测定法（ELISA）来检测Der p 1变应原。结果表明，这些单克隆抗体可应用于亲和柱以纯化天然Der p 1抗原，也可应用于夹心ELISA进行流行病学研究。
