Davies M W, Mehr S, Garland S T, Morley C J
Division of Neonatal Services, Division of Laboratory Services, Royal Women's Hospital, Melbourne, Australia.
Pediatrics. 2000 Aug;106(2):E18. doi: 10.1542/peds.106.2.e18.
To investigate the bacteria and fungi contaminating toys in neonatal intensive care unit (NICU) cots, the colonization rates, and factors that influence them.
A cross-sectional, longitudinal bacteriologic survey of all toys in the cots of infants in an NICU. All the toys in an infant's cot were cultured weekly for 4 weeks. Data were collected on the infant's postnatal age, the type of cot, whether humidity was added, characteristics of the toy, and any infant infections.
Over the 4-week period, there were 86 cultures from 34 toys of 19 infants. Bacteria were grown from 84/86 (98%): 84 of the cultures grew coagulase-negative Staphylococcus, 50 Micrococcus sp, 21 Bacillus sp, 13 methicillin-resistant Staphylococcus aureus, 12 diphtheroids, 4 group B streptococcus, 3 S aureus, 3 nonhemolytic streptococci, 3 group D streptococci, 4 alpha-hemolytic streptococci, and 2 coliforms. None grew fungi. The colonization rate did not differ with cot type, presence of humidity, size of the toy, toy fiber length, or the fluffiness score. Eight (42%) of the infants had positive blood culture results and 5/8 of the isolates (63%) were of the same type as that colonizing their corresponding toy.
With time, all the toys in NICU cots became colonized with bacteria. Many were potentially pathogenic. Toys may be reservoirs for potential infantile nosocomial sepsis. infant, newborn, toys, infection, neonatal intensive care.
调查新生儿重症监护病房（NICU）婴儿床玩具上的细菌和真菌污染情况、定植率及其影响因素。
对某NICU婴儿床内所有玩具进行横断面纵向细菌学调查。每周对婴儿床内所有玩具进行一次培养，持续4周。收集婴儿的出生后年龄、婴儿床类型、是否添加湿度、玩具特征以及婴儿是否发生感染等数据。
在4周期间，对19名婴儿的34个玩具进行了86次培养。86次培养中有84次（98%）培养出细菌：84次培养出凝固酶阴性葡萄球菌、50次培养出微球菌属、21次培养出芽孢杆菌属、13次培养出耐甲氧西林金黄色葡萄球菌、12次培养出类白喉杆菌、4次培养出B组链球菌、3次培养出金黄色葡萄球菌、3次培养出非溶血性链球菌、3次培养出D组链球菌、4次培养出α溶血性链球菌、2次培养出大肠菌群。均未培养出真菌。定植率在婴儿床类型、是否有湿度、玩具大小、玩具纤维长度或蓬松度评分方面无差异。8名（42%）婴儿血培养结果呈阳性，其中5/8（63%）的分离株与定植在其相应玩具上的细菌类型相同。
随着时间推移，NICU婴儿床内所有玩具都被细菌定植。许多细菌具有潜在致病性。玩具可能是婴儿医院感染性败血症的潜在来源。婴儿、新生儿、玩具、感染、新生儿重症监护。
