Neonatal intensive care unit: reservoirs of nosocomial pathogens.

Improvement in the care and treatment of neonates had contributed to their increased survival. Nosocomial infection remains an important problem in intensive care units. Hospital wards had been shown to act as reservoirs of pathogenic microorganisms associated with infection. To assess the prevalence of pathogenic organisms in the environment of the neonatal unit, 92 swabs were randomly collected from cots, incubators and various equipments in the unit and were cultured on Blood agar and MacConkey agar plates. Air contamination was detected by exposing the same types of agar plates for 3 hours in several areas of the unit. After 48 hours incubation, isolates were identified biochemically. There is marked congestion in the unit. Ninety one percent of swabs yielded growth, with coagulase negative Staphylococcus being the predominant organism (44%), followed by Bacillus species (20%), E. coli (12.5%), and Klebsiella (8.5%), Pseudomonas species (3.6%) and moulds (3.6%). Sedimentation plates had colony counts of from 10 - 100 per plate and the majority of the cultures were polymicrobial cultures. The presence of various Gram-negative bacili including known neonatal pathogens (like E. Coli and Pseudomonas) especially on ward equipment and congestion in the ward has the potential to cause nosocomial infection.