Boggess K A, Kay H H, Crapo J D, Moore W F, Suliman H B, Oury T D
Departments of Obstetrics and Gynecology, Duke University Medical Center, Durham, NC, USA.
Am J Obstet Gynecol. 2000 Jul;183(1):199-205. doi: 10.1067/mob.2000.105426.
The aim of this study was to determine placental localization and activity of extracellular superoxide dismutase, a nitric oxide modulator, during early gestation and to correlate these characteristics with fetal vascular development.
First-trimester (n = 10) and second-trimester (n = 10) villi were obtained at elective pregnancy termination. Extracellular superoxide dismutase was localized by means of an immunoperoxidase method. Activity was measured by determining the inhibition of cytochrome c reduction at pH 10 and messenger ribonucleic acid expression by in situ hybridization.
Extracellular superoxide dismutase was intracellular within villous trophoblasts until 17 weeks' gestation, when it relocated to the villous extracellular matrix. Activities were similar between first- and second-trimester villi. In situ hybridization confirmed extracellular superoxide dismutase messenger ribonucleic acid within trophoblasts throughout gestation.
Extracellular superoxide dismutase is produced by trophoblasts early in pregnancy, but it remains intracellular until 17 weeks' gestation, which may be related to fetal vascular development.
本研究的目的是确定妊娠早期作为一氧化氮调节剂的细胞外超氧化物歧化酶的胎盘定位和活性，并将这些特征与胎儿血管发育相关联。
在选择性终止妊娠时获取孕早期（n = 10）和孕中期（n = 10）的绒毛。采用免疫过氧化物酶法对细胞外超氧化物歧化酶进行定位。通过测定pH 10时细胞色素c还原的抑制作用来测量活性，并通过原位杂交检测信使核糖核酸表达。
细胞外超氧化物歧化酶在妊娠17周前位于绒毛滋养层细胞内，之后转移至绒毛细胞外基质。孕早期和孕中期绒毛的活性相似。原位杂交证实在整个妊娠期滋养层细胞内存在细胞外超氧化物歧化酶信使核糖核酸。
细胞外超氧化物歧化酶在妊娠早期由滋养层细胞产生，但在妊娠17周前一直位于细胞内，这可能与胎儿血管发育有关。
