[Mutations of APC gene MCR region in sporadic colorectal adenomas and carcinomas].

OBJECTIVE

Fifteen specimens of colorectal adenomas and 45 specimens of carcinomas and each of their normal mucosa were analyzed for the APC gene exon 15 MCR(mutation cluster region).

METHODS

Gene mutation was examined by PCR-SSCP technique.

RESULTS

In three out of 15 cases of adenomas(20.0%) and 10 out of 45 cases of carcinomas(22.2%), mutations at MCR was detected. In colorectal carcinoma, APC gene mutation was not related to size, location, histologic features and lymph node metastases. In MCR region, 1260-1359 codons gave a lowest mutation frequency, while 1339-1436 codons showed the highest mutation frequency.

CONCLUSION

APC gene mutation detected in colorecral adenomas indicates that the APC gene mutation is an early event in colorectal carcinogenesis. It helps early diagnosis of colorectal carcinoma.