[PCNA反义RNA表达载体的构建及其对人胃癌细胞的抗肿瘤作用]
[Construction of PCNA antisense RNA expression vector and its antitumorigenic effect on human gastric cancer cell].
作者信息
Dai L, Zhang X, Hui H
机构信息
Department of Gastroenterology, 210 Hospital of PLA, Dalian.
出版信息
Zhonghua Zhong Liu Za Zhi. 1998 Sep;20(5):337-9.
OBJECTIVE
To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell.
METHODS
An eukaryotic expression vector pDR-PCNA with human PCNA gene insert in reverse direction was successfully constructed. The human gastric cancer cell line SGC-7901 was transfected with pDR-PCNA by lipofectamine.
RESULTS
Growth rate, protein and RNA biosynthesis and DNA contents in S-phase and G2/M-phase of the transfected SGC-7901 were significantly suppressed in comparison with those of the parental cell line.
CONCLUSION
PCNA antisense RNA can significantly suppress the in vitro growth of gastric cancer cell line.
目的
观察增殖细胞核抗原（PCNA）反义RNA对人胃癌细胞生长特性的影响。
方法
成功构建了反向插入人PCNA基因的真核表达载体pDR-PCNA。采用脂质体介导法将pDR-PCNA转染人胃癌细胞系SGC-7901。
结果
与亲代细胞系相比，转染后的SGC-7901细胞的生长速率、蛋白质和RNA生物合成以及S期和G2/M期的DNA含量均受到显著抑制。
结论
PCNA反义RNA可显著抑制胃癌细胞系的体外生长。
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