[Construction of PCNA antisense RNA expression vector and its antitumorigenic effect on human gastric cancer cell].

OBJECTIVE

To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell.

METHODS

An eukaryotic expression vector pDR-PCNA with human PCNA gene insert in reverse direction was successfully constructed. The human gastric cancer cell line SGC-7901 was transfected with pDR-PCNA by lipofectamine.

RESULTS

Growth rate, protein and RNA biosynthesis and DNA contents in S-phase and G2/M-phase of the transfected SGC-7901 were significantly suppressed in comparison with those of the parental cell line.

CONCLUSION

PCNA antisense RNA can significantly suppress the in vitro growth of gastric cancer cell line.