[肾母细胞瘤基因（WT1）在白血病中的表达及其临床意义]

[Expression of Wilms' tumor gene (WT1) in leukemias and its clinical implication].

作者信息

Wu X, Wang Y, Pei X

机构信息

General Hospital of PLA, Beijing.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 1998 Jan;19(1):16-9.

PMID:10921096

Abstract

OBJECTIVE

To elucidate the expression of Wilms' tumor gene (WT1) in leukemias and its clinical implication.

METHODS

Expression of WT1 mRNA was detected in four leukemia cell lines, 25 AML patients, 11 ALL patients, 9 CML patients, 15 healthy subjects and 10 non-blood diseases patients by using RT-PCR.

RESULTS

WT1 mRNA expression was revealed in K562, HL-60 and TF-1 cell lines, 21 of 25 (84%) AML patients, 8 of 11 (73%) ALL patients, 2 CML patients in blast crisis and 1 of 7 CML patients in accelerated and chronic phase. WT1 mRNA was undetectable in PBMNC from 15 healthy subjects and in bone marrow cells from 10 non-blood diseases patients.

CONCLUSION

WT1 gene is expressed in the majority of acute leukemias and thus is involved in human leukemogenesis. WT1 is also a new important marker for monitoring MRD in acute leukemias.

摘要

目的

阐明肾母细胞瘤基因（WT1）在白血病中的表达及其临床意义。

方法

采用逆转录聚合酶链反应（RT-PCR）检测4种白血病细胞系、25例急性髓系白血病（AML）患者、11例急性淋巴细胞白血病（ALL）患者、9例慢性髓系白血病（CML）患者、15名健康受试者及10例非血液系统疾病患者中WT1 mRNA的表达。

结果

在K562、HL-60和TF-1细胞系中，25例AML患者中的21例（84%）、11例ALL患者中的8例（73%）、2例处于急变期的CML患者以及7例处于加速期和慢性期的CML患者中的1例检测到WT1 mRNA表达。在15名健康受试者的外周血单个核细胞（PBMNC）和10例非血液系统疾病患者的骨髓细胞中未检测到WT1 mRNA。