Brody J I, Burningham R A, Nowell P C, Rowlands D T, Freiburg P, Daniele R P
Am J Med. 1975 Apr;58(4):547-52. doi: 10.1016/0002-9343(75)90129-1.
An inappropriate, sustained and absolute lymphocytosis in a young man was investigated using technics which attempted to define the more detailed features of these cells and their T and B subpopulations. The results of the tests for lymphocyte RFC (E) and complement (EAC) rosette formation, immunofluorescence, in vitro phytohemagglutin (PHA) stimulation and 14C-cyclo-phosphamide binding assays, when evaluated in a combined and interrelated fashion, indicated that the lymphocytosis was mainly but not solely, due to an absolute elevation in circulating T lymphocytes. Moreover, an aneuploid cell line with 47 chromosomes, presumably T cells, also was detected in PHA-stimulated cultures. These data, associated with the patient's anemia, lymphocyte-infiltrated bone marrow, hepatosplenomegaly, pharyngeal lymphoid tumor and the clonal proliferation of lymphocytes bearing an abnormal karyotype, furnish evidence that the lymphocytosis is more than an unremitting leukemoid reaction and is potentially neoplastic. The detection of the chromosomal abnormality at this patient's age and point in disease may make him especially unique in that he could be a clinical prototype for other patients with lymphoreticular tumors which remain undetected at their onset.
对一名年轻男性不适当的、持续性的绝对淋巴细胞增多症进行了研究，采用了一些技术来试图明确这些细胞及其T和B亚群的更详细特征。当以综合和相互关联的方式评估淋巴细胞RFC（E）和补体（EAC）花环形成试验、免疫荧光、体外植物血凝素（PHA）刺激试验以及14C - 环磷酰胺结合试验的结果时，表明淋巴细胞增多主要但并非仅仅是由于循环T淋巴细胞的绝对升高。此外，在PHA刺激的培养物中还检测到了一个具有47条染色体的非整倍体细胞系，推测为T细胞。这些数据，结合患者的贫血、淋巴细胞浸润的骨髓、肝脾肿大、咽部淋巴肿瘤以及具有异常核型的淋巴细胞的克隆增殖，证明淋巴细胞增多不仅仅是一种持续的类白血病反应，而且具有潜在的肿瘤性。在该患者的年龄和疾病阶段检测到染色体异常可能使他尤为独特，因为他可能是其他淋巴网状肿瘤患者在发病时未被发现的临床原型。
