Han J H, Lee S H, Tan Y Q, LeMosy E K, Hashimoto C
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA.
Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):9093-7. doi: 10.1073/pnas.97.16.9093.
The dorsoventral axis of the Drosophila embryo is induced by a ventrally restricted ligand for the receptor Toll. The Toll ligand is generated by a proteolytic processing reaction, which occurs at the end of a proteolytic cascade and requires the gastrulation defective (gd), nudel, pipe, and windbeutel genes. Here we demonstrate that the GD protein is a serine protease and that the three other genes act to restrict GD activity to the ventral side of the embryo. Our data support a model in which the GD protease catalyzes the ventral activation of the proteolytic cascade that produces the Toll ligand.
果蝇胚胎的背腹轴是由受体Toll的一种腹侧受限配体诱导形成的。Toll配体是通过蛋白水解加工反应产生的,该反应发生在蛋白水解级联反应的末端,需要原肠胚形成缺陷(gd)、nudel、管道(pipe)和风袋(windbeutel)基因。在这里,我们证明GD蛋白是一种丝氨酸蛋白酶,并且其他三个基因的作用是将GD活性限制在胚胎的腹侧。我们的数据支持一种模型,即GD蛋白酶催化产生Toll配体的蛋白水解级联反应的腹侧激活。