Pucharcos C, Estivill X, de la Luna S
Down Syndrome Research Group, Medical and Molecular Genetics Center, IRO, Hospital Duran i Reynals, Avia. de Castelldefels Km 2.7, L'Hospitalet de Llobregat, 08907, Barcelona, Spain.
FEBS Lett. 2000 Jul 28;478(1-2):43-51. doi: 10.1016/s0014-5793(00)01793-2.
Intersectin 1 (ITSN1) is a binding partner of dynamin that has been shown to participate in clathrin-mediated endocytosis. Here we report the characterization of a new human gene, ITSN2, highly similar to ITSN1. Alternative splicing of ITSN2 generates a short isoform with two EH domains, a coiled-coil region and five SH3 domains, and a longer isoform containing extra carboxy domains (DH, PH and C2 domains), suggesting that it could act as a guanine nucleotide exchange factor for Rho-like GTPases. ITSN2 expression analysis indicates that it is widely expressed in human tissues. Intersectin 2 isoforms show a subcellular distribution similar to other components of the endocytic machinery and co-localize with Eps15. Moreover, their overexpression, as well as the corresponding ITSN1 protein forms, inhibits transferrin internalization.
相交蛋白1（ITSN1）是发动蛋白的结合伴侣，已被证明参与网格蛋白介导的内吞作用。在此，我们报告了一个与ITSN1高度相似的新人类基因ITSN2的特征。ITSN2的可变剪接产生一种短异构体，其具有两个EH结构域、一个卷曲螺旋区域和五个SH3结构域，以及一种包含额外羧基结构域（DH、PH和C2结构域）的更长异构体，这表明它可能作为Rho样GTP酶的鸟嘌呤核苷酸交换因子发挥作用。ITSN2表达分析表明它在人类组织中广泛表达。相交蛋白2异构体显示出与内吞机制的其他组分相似的亚细胞分布，并与Eps15共定位。此外，它们的过表达以及相应的ITSN1蛋白形式均抑制转铁蛋白的内化。
