在大鼠肝脏中，肝细胞核因子（HNF）-4α基因被糖皮质激素和胰高血糖素激活，并被胰岛素抑制。

The gene for hepatocyte nuclear factor (HNF)-4alpha is activated by glucocorticoids and glucagon, and repressed by insulin in rat liver.

作者信息

Oyadomari S, Matsuno F, Chowdhury S, Kimura T, Iwase K, Araki E, Shichiri M, Mori M, Takiguchi M

机构信息

Department of Molecular Genetics, Kumamoto University School of Medicine, Honjo 2-2-1, Kumamoto 860-0811, Japan.

出版信息

FEBS Lett. 2000 Jul 28;478(1-2):141-6. doi: 10.1016/s0014-5793(00)01840-8.

DOI:10.1016/s0014-5793(00)01840-8

PMID:10922486

Abstract

The gene for a transcription factor hepatocyte nuclear factor-4alpha (HNF-4alpha) is responsible for maturity-onset diabetes of the young, type 1. We examined hormonal regulation of the HNF-4alpha gene in the liver. Stimulation of primary-cultured rat hepatocytes with dexamethasone or glucagon led to induction of HNF-4alpha mRNA, being antagonized by insulin. In the liver of streptozotocin-induced diabetic rat, mRNA and protein levels for HNF-4alpha were elevated, and were normalized by insulin treatment. Therefore, HNF-4alpha in the liver is likely to be involved in the regulation of glucose metabolism in response to these hormones.

摘要

转录因子肝细胞核因子4α（HNF - 4α）的基因与1型青年发病型糖尿病有关。我们研究了肝脏中HNF - 4α基因的激素调节。用地塞米松或胰高血糖素刺激原代培养的大鼠肝细胞会导致HNF - 4α mRNA的诱导，而胰岛素会拮抗这种诱导作用。在链脲佐菌素诱导的糖尿病大鼠肝脏中，HNF - 4α的mRNA和蛋白水平升高，而胰岛素治疗可使其恢复正常。因此，肝脏中的HNF - 4α可能参与了对这些激素作出反应的葡萄糖代谢调节。