David A, Bernard J, Thiernesse N, Nicolas G, Cerottini J C, Zagury D
C R Seances Acad Sci D. 1979 Jan 29;288(4):441-4.
In this paper, we formulate the hypothesis that in the process of target cell lysis a lysosomal enzyme regurgitation, performed by killer cells at the level of the target effector junction, accounts for the target lesion which precedes the lysis (lethal hit). This process of exocytosis, similar to the one described previously in polymorphonuclear neutrophils is supported by cytological studies performed directly on identified killers isolated by micromanipulation. Light and electron microscopy observations confirm a previous report which describes the effector cells rich in lysosomal bodies. In addition, when a killer cell is associated with a target cell to form a conjugate, lysosomes are concentrated near the cell junction and, after incubation at 37 degrees C, acid phosphatases may be detected at the junction. Lysosomal enzyme exocytosis explains why target lysis needs an effector target binding to occur and also the other conditions required for any exocytosis process such as Ca++ in the medium, integrity of the microtubular apparatus, a low level of cyclic AMP and energy dependancy.
在本文中，我们提出这样一个假说：在靶细胞裂解过程中，杀伤细胞在靶效应连接部位进行的溶酶体酶反流，是裂解（致死性打击）之前靶损伤的原因。这种胞吐过程类似于先前在多形核中性粒细胞中所描述的过程，通过对经显微操作分离出的已鉴定杀伤细胞直接进行的细胞学研究得到了支持。光学显微镜和电子显微镜观察结果证实了先前一篇描述富含溶酶体小体的效应细胞的报道。此外，当一个杀伤细胞与一个靶细胞结合形成共轭体时，溶酶体集中在细胞连接处附近，并且在37℃孵育后，在连接处可检测到酸性磷酸酶。溶酶体酶胞吐作用解释了为什么靶裂解需要效应细胞与靶细胞结合才能发生，以及胞吐过程所需的其他条件，如培养基中的钙离子、微管装置的完整性、低水平的环磷酸腺苷和能量依赖性。
