Zhou S, Mao N, Zhao M
Institute of Basic Medical Sciences, Academy of Military Medical Sciences Beijing.
Zhonghua Yi Xue Za Zhi. 1998 Jan;78(1):37-9.
To determine the effects of recombinant human FLT3 ligand (rhFL) on in vitro expansion of human cord blood CD34+ cells.
Human cord blood CD34+ cells were enriched by using a magnetic cell isolation system. CD34+ cells were incubated in liquid culture in the presence of different cytokines. The total number of all cells and the number of CD34+ cells and hematopoietic progenitor cells (CFU-GM and BFU-E) were counted at various time intervals.
Human cord blood CD34+ cells could be enriched to the purity of more than 80%. The number of CD34+ cells increased 3.4 folds in the presence of rhFL + IL3 + IL6 + GMCSF + EPO in seven days, while in the control group without rhFL, it increased 1.5 folds. The difference of the number of more matured hematopoietic progenitor cells in the CD34+ cell population between the control group and the rhFL group was not significant.
Only the primitive hematopoietic progenitor cells are expanded by rhFL according to the results obtained in the exclusion method.
确定重组人FLT3配体（rhFL）对人脐血CD34+细胞体外扩增的影响。
使用磁性细胞分离系统富集人脐血CD34+细胞。将CD34+细胞在不同细胞因子存在的情况下进行液体培养。在不同时间间隔对所有细胞总数、CD34+细胞数以及造血祖细胞（CFU-GM和BFU-E）数进行计数。
人脐血CD34+细胞可富集至纯度超过80%。在rhFL + IL3 + IL6 + GMCSF + EPO存在的情况下，CD34+细胞数量在7天内增加了3.4倍，而在无rhFL的对照组中增加了1.5倍。对照组和rhFL组之间CD34+细胞群体中更成熟造血祖细胞数量的差异不显著。
根据排除法获得的结果，只有原始造血祖细胞可被rhFL扩增。
