Moran D L
Ohio University, Department of Environmental and Plant Biology, 317 Porter Hall, Athens, OH 45701, USA.
Gene. 2000 Jul 25;253(1):19-29. doi: 10.1016/s0378-1119(00)00222-5.
The eukaryotic ribosomal protein (RP) L9 is highly conserved in nature, and its gene is expressed to high levels in the actively growing tissues of pea. The transcriptional activity of the gene is highest in root, cambial and shoot meristems and immature tissues of the plant. Promoter deletion analysis using constructs employing the reporter gene gus were stably transferred into tobacco and revealed that the fully functional promoter is found in the first 316bp upstream from the start codon. Transgenic pea plants carrying one of these constructs show that translational efficiency mirrors gene transcription; gene expression appears to be developmentally regulated at the level of transcription. The coding region of the gene shares 80% amino acid homology with Arabidopsis and 76% homology with rice. Comparisons of the gene structure to that of the human, fruit fly, yeast, and Arabidopsis homologues reveal a close relationship in both promoter structure and intron insertion sites with the Arabidopsis gene. A nucleotide sequence alignment of the pea gene with other plant RP genes revealed that a sequence, -TTAGGGTTTT-, was commonly found in the forward and/or the inverted orientation at or near the TATA boxes of the promoters of these genes and may have a role in regulating the coordinate production of the RP genes in plants.
真核生物核糖体蛋白（RP）L9在自然界中高度保守，其基因在豌豆活跃生长的组织中高水平表达。该基因的转录活性在植物的根、形成层和茎尖分生组织以及未成熟组织中最高。使用携带报告基因gus的构建体进行的启动子缺失分析被稳定地转入烟草中，结果显示在起始密码子上游的前316bp中发现了功能完全的启动子。携带这些构建体之一的转基因豌豆植株表明，翻译效率反映了基因转录；基因表达似乎在转录水平上受到发育调控。该基因的编码区与拟南芥的氨基酸同源性为80%，与水稻的同源性为76%。将该基因的结构与人类、果蝇、酵母和拟南芥同源基因的结构进行比较，发现其启动子结构和内含子插入位点与拟南芥基因都有密切关系。豌豆基因与其他植物RP基因的核苷酸序列比对显示，在这些基因启动子的TATA框处或附近，通常能以正向和/或反向方向发现一个序列“-TTAGGGTTTT-”，它可能在调节植物中RP基因的协同产生方面发挥作用。
