Witherden D A, Boismenu R, Havran W L
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
J Immunol. 2000 Aug 15;165(4):1902-9. doi: 10.4049/jimmunol.165.4.1902.
We have examined the role of CD81 in the activation of murine splenic alphabeta T cells. Expression of the CD81 molecule on T cells increases following activation, raising the possibility of a role for this molecule in progression of the activation process. Using an in vitro costimulation assay, we show that CD81 can function as a costimulatory molecule on both CD4+ and CD8+ T cells. This costimulation functions independently of CD28, and unlike costimulation through CD28, is susceptible to inhibition by cyclosporin A. Strikingly, the pattern of cytokine production elicited by costimulation via CD81 is unique. IL-2 production was not up-regulated, whereas both IFN-gamma and TNF-alpha expression significantly increased. Together our results demonstrate an alternate pathway for costimulation of T cell activation mediated by CD81.
我们研究了CD81在小鼠脾脏αβ T细胞激活中的作用。T细胞激活后，CD81分子在T细胞上的表达增加，这增加了该分子在激活过程进展中发挥作用的可能性。使用体外共刺激试验，我们发现CD81可作为CD4+和CD8+ T细胞上的共刺激分子发挥作用。这种共刺激作用独立于CD28，并且与通过CD28的共刺激不同，它易受环孢素A的抑制。引人注目的是，通过CD81共刺激引发的细胞因子产生模式是独特的。IL-2的产生没有上调，而IFN-γ和TNF-α的表达均显著增加。我们的研究结果共同证明了由CD81介导的T细胞激活共刺激的另一条途径。
