Sung M W, Lee S G, Yoon S J, Lee H J, Heo D S, Kim K H, Koh T Y, Choi S H, Park S W, Koo J W, Kwon T Y
Department of Otolaryngology, Seoul National University College of Medicine, Korea.
Anticancer Res. 2000 May-Jun;20(3A):1653-6.
The effect of combining adenoviral vector and cationic liposomes on the efficiency of gene transfer to head and neck tumor cells was investigated. Two human and two murine cell lines were used for the screening of gene transfer efficiency using an adenoviral vector. Cationic liposome-enhanced gene transfer was checked using a murine squamous carcinoma cell line, SCCVII/SF. A considerable difference in the efficiency of gene transduction was observed among the cell lines. The combination of DOSPER and adenoviral vector containing human alkaline phosphatase showed a remarkable enhancing effect in gene transfer in vitro and in vivo, compared to the adenovirus alone or control groups. With an improvement in the efficiency of gene transfer, it may be possible not only to enhance the expression of transduced genes, but also to deliver a smaller amount of virus, as a result, reducing toxicity and the immune response against adenovirus.
研究了腺病毒载体与阳离子脂质体联合使用对基因转导至头颈肿瘤细胞效率的影响。使用两种人类细胞系和两种小鼠细胞系，通过腺病毒载体筛选基因转导效率。使用小鼠鳞状癌细胞系SCCVII/SF检测阳离子脂质体增强的基因转导。在各细胞系中观察到基因转导效率存在显著差异。与单独使用腺病毒或对照组相比，DOSPER与含人碱性磷酸酶的腺病毒载体联合使用在体外和体内基因转导中均显示出显著的增强作用。随着基因转导效率的提高，不仅有可能增强转导基因的表达，还可能减少病毒用量，从而降低毒性和针对腺病毒的免疫反应。
