Favy D A, Rio P G, Vissac C, Maurizis J C, Bignon Y J, Bernard-Gallon D J
Laboratoire d'Oncologie Moléculaire, INSERM CRI 9502, Clermont-Ferrand, France.
Anticancer Res. 2000 May-Jun;20(3A):1703-4.
Previously, we reported experimental evidence that BRCA1, breast and ovarian cancer susceptibility gene is up-regulated in response to Prolactin stimulation. In this work, we studied the effects of Cyclosporine A and the competition with Prolactin on BRCA1 protein expression in vitro.
The expression of BRCA1 was monitored in a human breast cancer cell line (MCF7) by comparison with a normal breast epithelial one (MCF10a) treated with Cyclosporine A and ovine Prolactin. The amount of BRCA1 protein expression was quantified using a strategy of two successive affinity perfusion chromatographies.
We showed that Prolactin in presence of Cyclosporine A has no effect on BRCA1 protein expression in human breast cell lines. This emphasized the hypothesis that BRCA1 may be stimulated by Prolactin.
此前，我们报道了实验证据，即乳腺癌和卵巢癌易感基因BRCA1在催乳素刺激下上调。在这项工作中，我们研究了环孢素A的作用以及它与催乳素竞争对体外BRCA1蛋白表达的影响。
通过与用环孢素A和羊催乳素处理的正常乳腺上皮细胞系（MCF10a）比较，监测人乳腺癌细胞系（MCF7）中BRCA1的表达。使用连续两次亲和灌注色谱法策略对BRCA1蛋白表达量进行定量。
我们发现，在环孢素A存在的情况下，催乳素对人乳腺细胞系中BRCA1蛋白表达没有影响。这强化了BRCA1可能受催乳素刺激的假说。
