Makino M, Yamane N, Taniguchi T, Honboh T, Kurayoshi K, Kaibara N
First Department of Surgery, Tottori University, Faculty of Medicine, Yonago, Japan.
Anticancer Res. 2000 May-Jun;20(3B):2055-9.
We examined whether overexpression of p53 can be used as a new genetic marker to predict the presence of lymph node metastases of early invasive colorectal cancer. Forty-nine patients with primary colorectal adenocarcinomas invading to the submucosa (sm-CRC) were analyzed and 7 patients were found to have lymph node metastases. Immunostaining was used to detect the p53 overexpression; 43% of sm-CRC stained positive for p53 and all the cancer cells metastasized to lymph nodes were p53 positive. Both lymph node involvement and tumor budding were significantly more frequent in p53 positive than p53 negative tumors (p < 0.05, respectively), and multivariate analysis showed that p53 overexpression constituted a higher relative lisk for lymph node metastases of sm-CRC than either histologic type, level of sm invasion, macroscopic type, tumor budding or vascular invasion, although the difference was not significant (p = 0.086). We concluded that p53 overexpression is a useful biological marker of lymph node metastases of sm-CRC, and that p53 negative status may be an indicator for limited surgery, such as local excision of sm-CRC.
我们研究了p53过表达是否可作为一种新的基因标志物，用于预测早期浸润性结直肠癌淋巴结转移的存在。对49例原发性结直肠腺癌侵犯至黏膜下层（sm-CRC）的患者进行了分析，发现7例有淋巴结转移。采用免疫染色检测p53过表达；43%的sm-CRC p53染色呈阳性，且所有转移至淋巴结的癌细胞p53均为阳性。p53阳性肿瘤的淋巴结受累和肿瘤芽生均显著多于p53阴性肿瘤（分别为p<0.05），多因素分析显示，p53过表达构成sm-CRC淋巴结转移的相对风险高于组织学类型、sm浸润深度、大体类型、肿瘤芽生或血管侵犯，尽管差异不显著（p=0.086）。我们得出结论，p53过表达是sm-CRC淋巴结转移的一个有用的生物学标志物，p53阴性状态可能是有限手术（如sm-CRC局部切除）的一个指标。
