Horch R E, Andree C, Kopp J, Tánczos E, Voigt M, Bannasch H, Walgenbach K J, Dai F P, Bittner K, Galla T J, Stark G B
Abteilung Plastische und Handchirurgie, Albert-Ludwigs-Universität Freiburg.
Zentralbl Chir. 2000;125 Suppl 1:74-8.
A variety of reasons can afflict wound healing. Current research is focussed on the acceleration of wound healing by stimulating molecular processes. Gene therapy may offer completely new ways to treat chronic wounds. Possible advantages of gene therapeutic modulation of wound healing might be a long term efficiency, systemic or local regulation of gene expression and low side-effects. Current goals comprise the improvement of transfection efficiency and specificity. In vivo applications are therefore focussed on optimized inducible or even cell-type specific promotors, as well as on improved local application techniques. Studies from our laboratory demonstrate the possibility to combine modern cell culture techniques with different types of gene transfer. This enables the simultaneous grafting of manipulated cells to the wound with the continuous delivery of specific proteins of interest. Experimentally, this lead to accelerated closure of partial and full thickness animal wounds. Clinically, gene therapy for the treatment of chronic wounds seems to be a realistic goal within the next years and might be applicable for a variety of novel indications.
多种原因会影响伤口愈合。当前的研究集中在通过刺激分子过程来加速伤口愈合。基因治疗可能会提供全新的慢性伤口治疗方法。基因治疗调控伤口愈合的潜在优势可能在于长期有效性、基因表达的全身或局部调节以及低副作用。当前的目标包括提高转染效率和特异性。因此，体内应用聚焦于优化的可诱导甚至细胞类型特异性启动子，以及改进的局部应用技术。我们实验室的研究表明，有可能将现代细胞培养技术与不同类型的基因转移相结合。这使得能够将经过处理的细胞同时移植到伤口，并持续递送感兴趣的特定蛋白质。在实验中，这导致部分厚度和全厚度动物伤口加速愈合。在临床上，治疗慢性伤口的基因治疗在未来几年似乎是一个现实的目标，并且可能适用于多种新的适应症。
