一种用于检测乳酸的蛋白质生物传感器。
A protein biosensor for lactate.
作者信息
D'Auria S, Gryczynski Z, Gryczynski I, Rossi M, Lakowicz J R
机构信息
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 21201, USA.
出版信息
Anal Biochem. 2000 Jul 15;283(1):83-8. doi: 10.1006/abio.2000.4611.
Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for L-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactate-sensing protein.
血液乳酸是一种具有临床价值的诊断指标。在本初步报告中，我们描述了一种基于牛心乳酸脱氢酶（LDH）的L-乳酸蛋白质生物传感器。LDH用8-苯胺基-1-萘磺酸（ANS）进行非共价标记。结合乳酸后，ANS标记的LDH发射强度降低了约40%。这种降低可用于测量乳酸浓度。ANS标记的LDH进一步用于一种新的传感形式——偏振传感，这种传感形式适合小型化至即时检测乳酸监测仪。然而，牛心LDH的时间稳定性表明，在开发更稳定的乳酸传感蛋白之前，需要进一步进行蛋白质工程。
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