CpG DNA作为一种促进Th1型免疫反应的佐剂用于克氏锥虫免疫接种。
CpG DNA as a Th1-promoting adjuvant in immunization against Trypanosoma cruzi.
作者信息
Corral R S, Petray P B
机构信息
Laboratorio de Virología, Hospital de Niños Ricardo Gutierrez, Gallo 1330, 1425, Buenos Aires, Argentina.
出版信息
Vaccine. 2000 Sep 15;19(2-3):234-42. doi: 10.1016/s0264-410x(00)00172-9.
Th1-type immune response plays a critical role in resistance to Trypanosoma cruzi infection. We asked whether a synthetic oligodeoxynucleotide that contains immunostimulatory CpG motifs (CpG ODN), known to promote a Th1 response, could act as an adjuvant in immunization with parasite antigens. Mice immunized with a whole homogenate (WH) of T. cruzi antigens co-administered with CpG ODN presented high titers of T. cruzi antibodies (IgG2a isotype), strong delayed type hypersensitivity and a Th1-dominated (IFN-gamma and IL-12) cytokine profile. Furthermore, WH plus CpG ODN protected mice from challenge with an otherwise lethal dose of bloodstream trypomastigotes. As reported for leishmaniasis and malaria, CpG ODN holds considerable promise as an adjuvant for future vaccines against T. cruzi.
1型辅助性T细胞（Th1）介导的免疫反应在抵抗克氏锥虫感染中起着关键作用。我们探讨了一种含有免疫刺激CpG基序的合成寡脱氧核苷酸（CpG ODN），已知其能促进Th1反应，是否可作为寄生虫抗原免疫接种的佐剂。用克氏锥虫抗原全匀浆（WH）与CpG ODN共同免疫的小鼠呈现出高滴度的克氏锥虫抗体（IgG2a亚型）、强烈的迟发型超敏反应以及以Th1为主导（干扰素-γ和白细胞介素-12）的细胞因子谱。此外，WH加CpG ODN可保护小鼠免受致死剂量的血流型锥鞭毛体攻击。正如利什曼病和疟疾的报道一样，CpG ODN作为未来抗克氏锥虫疫苗的佐剂具有很大的前景。
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