Vagner-Capodano A M, Noel B, Stahl A
Pathol Biol (Paris). 1975 Feb;23(2):119-32.
Fluorescent techniques permitted us to draw up a system of banding of human chromosomes, using quinacrine derivatives. This procedure may be used to measure the density of the chromosomes. Recently, other techniques which use various fluorochromes after denaturation, have been developed. The latter, which use acridine orange, give remarkably reliable and reproducible results. These techniques are particularly useful for the analysis of structural changes in the chromosomes. Their use is illustrated by a certain number of observations where they permitted the authors to determine the type of structural change and identify the chromosomes involved. The method of constitution and significance of the fluorescent bands is very controversial. It is not clearly known whether the fluorochromes combine with DNA or with protein bound to the latter. In spite of this uncertainty, the existence of heterogeneity in the structure of the chromatids at the metaphase provides new information for the understanding of chromosome organisation.
荧光技术使我们能够利用喹吖因衍生物绘制人类染色体的带型系统。这一程序可用于测量染色体的密度。最近，还开发了其他在变性后使用各种荧光染料的技术。后者使用吖啶橙，能给出非常可靠且可重复的结果。这些技术对于分析染色体的结构变化特别有用。一定数量的观察结果说明了它们的用途，在这些观察中，作者能够确定结构变化的类型并识别出涉及的染色体。荧光带的构成方法和意义极具争议。目前尚不清楚荧光染料是与DNA结合，还是与与DNA结合的蛋白质结合。尽管存在这种不确定性，但中期染色单体结构的异质性为理解染色体组织提供了新的信息。
