Use of 2-methylpropan-2-ol to inhibit proteolysis and other protein interactions in a tissue homogenate: an illustrative application to the extraction of cathepsins B and L from liver tissue.
作者信息
Dennison C, Moolman L, Pillay C S, Meinesz R E
机构信息
School of Molecular and Cellular Biosciences, University of Natal, Pietermaritzburg 3209, South Africa.
出版信息
Anal Biochem. 2000 Aug 15;284(1):157-9. doi: 10.1006/abio.2000.4673.