一种使用顺式连接单纯疱疹病毒胸苷激酶对转基因进行无创成像的通用方法。
A general approach to the non-invasive imaging of transgenes using cis-linked herpes simplex virus thymidine kinase.
作者信息
Tjuvajev J G, Joshi A, Callegari J, Lindsley L, Joshi R, Balatoni J, Finn R, Larson S M, Sadelain M, Blasberg R G
机构信息
Department of Neurology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
出版信息
Neoplasia. 1999 Oct;1(4):315-20. doi: 10.1038/sj.neo.7900053.
Abstract
Non-invasive imaging of gene expression opens new prospects for the study of transgenic animals and the implementation of genetically based therapies in patients. We have sought to establish a general paradigm to enable whole body non-invasive imaging of any transgene. We show that the expression and imaging of HSV1-tk (a marker gene) can be used to monitor the expression of the LacZ gene (a second gene) under the transcriptional control of a single promoter within a bicistronic unit that includes a type II internal ribosomal entry site. In cells bearing a single copy of the vector, the expression of the two genes is proportional and constant, both in vitro and in vivo. We demonstrate that non-invasive imaging of HSV1-tk gene accurately reflects the topology and activity of the other cis-linked transgene.
摘要
基因表达的非侵入性成像为转基因动物的研究以及基于基因的疗法在患者中的应用开辟了新前景。我们试图建立一种通用模式，以实现对任何转基因的全身非侵入性成像。我们表明，单纯疱疹病毒1型胸苷激酶（一种标记基因）的表达和成像可用于监测在包含II型内部核糖体进入位点的双顺反子单元中单个启动子转录控制下的LacZ基因（另一个基因）的表达。在携带单拷贝载体的细胞中，这两个基因的表达在体外和体内均呈比例且恒定。我们证明，单纯疱疹病毒1型胸苷激酶基因的非侵入性成像准确反映了其他顺式连接转基因的拓扑结构和活性。
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