Valero M A, Varea M T, Marín R
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Spain.
Parasitol Res. 2000 Jul;86(7):558-62. doi: 10.1007/s004360000201.
A study was done of the possible association between the development of common bile-duct stones and the presence of worms in rats experimentally infected with Fasciola hepatica. A total of 157 rats were individually infected with 20 metacercariae, and another 40 animals served as controls. The rats were dissected at 100, 200, 300, and 400 days postinfection (p.i.). A significant association was observed between the observation of stones and the presence of F. hepatica adults. The global frequency of bile-duct lithiasis in the parasitized rats was 22%, with a significantly lower incidence being observed in the younger group (100 days p.i.). Different analytical techniques were used to determine the main stone components. Energy-dispersive X-ray microanalysis showed calcium to be the main component in all cases (82-94%). Scanning electron microscopy, nuclear magnetic resonance ([1H]- and [13C]-NMR), and mass spectrometry revealed the predominance of palmitic (C-16) and stearic (C-18) acid.
一项关于在实验性感染肝片吸虫的大鼠中胆总管结石形成与蠕虫存在之间可能关联的研究。总共157只大鼠分别感染20个囊蚴，另外40只动物作为对照。在感染后100、200、300和400天对大鼠进行解剖。观察到结石与肝片吸虫成虫的存在之间存在显著关联。受寄生大鼠中胆管结石症的总体发生率为22%，在较年轻组（感染后100天）观察到的发生率显著较低。使用不同的分析技术来确定结石的主要成分。能量色散X射线微分析表明在所有病例中钙是主要成分（82 - 94%）。扫描电子显微镜、核磁共振（[1H]-和[13C]-NMR）以及质谱分析显示棕榈酸（C - 16）和硬脂酸（C - 18）占主导地位。
