Zavala-Castro J E, Acosta-Viana K, Guzmán-Marín E, Rosado-Barrera M E, Rosales-Encina J L
Departamento de Patología experimental, Centro de Investigación y de Estudios Avanzados del I.P.N., Mexico City, Mexico.
Acta Trop. 2000 Sep 18;76(2):139-46. doi: 10.1016/s0001-706x(00)00079-6.
Knowledge regarding kinetoplast DNA organization in all members of the Trypanosomatid family is incomplete. Recently, the presence of kinetoplast-associated proteins in condensing kDNA networks in Crithidia fasciculata has been described and a role for these proteins in the maintenance of these complex structures was suggested. To investigate the presence of protein components in Trypanosoma cruzi kinetoplast, we previously described seven epimastigote kinetoplast-associated proteins. We report here the existence of kinetoplast binding proteins in amastigote and trypomastigote stages of T. cruzi, which could bind both mini and maxicircles components with a stage specific elements for every infective form of the parasite. We propose three major classes of kinetoplast-associated proteins related to the basic processes of this intricate disc structure and suggest a possible function of these binding proteins in the T. cruzi mitochondrial DNA organization.
关于锥虫科所有成员动质体DNA组织的知识并不完整。最近，已经描述了在克氏锥虫浓缩的动质体DNA网络中存在与动质体相关的蛋白质，并提出了这些蛋白质在维持这些复杂结构中的作用。为了研究克氏锥虫动质体中蛋白质成分的存在情况，我们之前描述了七种前鞭毛体动质体相关蛋白。我们在此报告，在克氏锥虫的无鞭毛体和 trypomastigote 阶段存在动质体结合蛋白，这些蛋白可以结合小环和大环成分，并且对于寄生虫的每种感染形式都具有阶段特异性元件。我们提出了与这种复杂盘状结构的基本过程相关的三类主要动质体相关蛋白，并提出了这些结合蛋白在克氏锥虫线粒体DNA组织中的可能功能。
