Shimotoyodome A, Meguro S, Hase T, Tokimitsu I, Sakata T
Biological Science Laboratories, Kao Corporation, 2606 Akabane, 321-3497 Ichikai-machi, Haga-gun, Tochigi, Japan.
Comp Biochem Physiol A Mol Integr Physiol. 2000 Jun;126(2):203-12. doi: 10.1016/s1095-6433(00)00194-x.
Constipation is a risk factor of colorectal cancer. Mucin is a major component of lumenal mucus, which protects the colorectal mucosa against mechanical and chemical damage. The aim of this study was to evaluate mucus production and to quantitate lumen mucus in a rat model of spastic constipation. We induced constipation with loperamide (1.5 mg/kg), and histochemically evaluated mucus production and the thickness of the mucus layer at the fecal surface. We quantitated the mucus attached to the mucosal surface using colonic perfusion with N-acetylcysteine. While more feces remained in the colon, there was less fecal excretion and lower fecal water content in loperamide-administered rats than in control rats. Crypt epithelial cells contained less mucus in constipated rats than in control rats. The mucus layer at the fecal surface was thinner and less mucus was recovered from the mucosal surface in constipated rats than in control rats. Mucus production of crypt epithelial cells and mucus at the fecal and mucosal surface were reduced by loperamide-induced constipation.
便秘是结直肠癌的一个风险因素。粘蛋白是肠腔黏液的主要成分，可保护结直肠黏膜免受机械和化学损伤。本研究的目的是评估痉挛性便秘大鼠模型中的黏液分泌情况并对肠腔黏液进行定量分析。我们用洛哌丁胺（1.5毫克/千克）诱导便秘，并通过组织化学方法评估黏液分泌情况以及粪便表面黏液层的厚度。我们使用N - 乙酰半胱氨酸进行结肠灌注，对附着在黏膜表面的黏液进行定量分析。与对照大鼠相比，给予洛哌丁胺的大鼠结肠内残留的粪便更多，粪便排泄量更少且粪便含水量更低。便秘大鼠的隐窝上皮细胞所含黏液比对照大鼠少。便秘大鼠粪便表面的黏液层更薄，从黏膜表面回收的黏液也比对照大鼠少。洛哌丁胺诱导的便秘会导致隐窝上皮细胞的黏液分泌以及粪便和黏膜表面的黏液减少。
