Sorimachi K, Cahnmann H J
Horm Metab Res. 1979 Mar;11(3):233-7. doi: 10.1055/s-0028-1092715.
Cultured monkey hepatocarcinoma cells were incubated with [3',5'-125I] diiodo-L--thyronine and with [3,5-125I] diiodo-L-thyronine. In both instances monodeiodination as well as sulfoconjugation took place. [3.-125I] iodothyronine and [3',5'-125I] diiodothyronine were identified as metabolites of [3'-5'-125I]-L-thyroxine in the cells, but neither [3-125I]-iodothyronine nor [3,5-125I] diiodothyronine was detected after incubation of the cells with ]3,5-125I]-L-thyroxine. No products of diphenyl ether splitting were observed in the medium after incubation of the cells with either [3,5-125I] diiodo-L-thyronine or [3,5-125I]-L-thyroxine.
将培养的猴肝癌细胞与[3',5'-125I]二碘-L-甲状腺原氨酸和[3,5-125I]二碘-L-甲状腺原氨酸一起孵育。在这两种情况下,均发生了单碘化作用以及硫酸化结合作用。[3'-125I]碘甲状腺原氨酸和[3',5'-125I]二碘甲状腺原氨酸被鉴定为细胞中[3'-5'-125I]-L-甲状腺素的代谢产物,但在用[3,5-125I]-L-甲状腺素孵育细胞后,未检测到[3-125I]-碘甲状腺原氨酸和[3,5-125I]二碘甲状腺原氨酸。在用[3,5-125I]二碘-L-甲状腺原氨酸或[3,5-125I]-L-甲状腺素孵育细胞后,在培养基中未观察到二苯醚裂解产物。