Mizugaki M, Ishizawa F, Yamazaki T, Hishinuma T
Department of Pharmaceutical Sciences, Tohoku University Hospital, 1-1, Seiryo-machi, Aoba-ku, 980-8574, Sendai, Japan.
Prostaglandins Other Lipid Mediat. 2000 Jul;62(2):157-64. doi: 10.1016/s0090-6980(00)00060-5.
We describe the effect of (-) epigallocatechin gallate (EGCg), one of catechins known in tea, on the prostacyclin (PGI) production by bovine aortic endothelial cells. The amounts of 6-keto-PGF(1alpha) and Delta(17)-6-keto-PGF(1alpha), stable metabolites of PGI(2) and PGI(3), released in culture medium were measured using gas chromatography/selected ion monitoring (GC/SIM). The prostacyclin production of endothelial cells was increased by EGCg in a dose- and time-dependent manner. The effect by EGCg was stronger than any other catechins (catechin, epicatechin, epigallocatechin, and epicatechin gallate). When endothelial cells incubated with EGCg and arachidonic acid (AA) or eicosapentaenoic acid (EPA), PGI(2), and PGI(3) production were increased greater than those incubated with AA or EPA alone. Furthermore, gallic acid, that also has a pyrogallol structure, increased PGI(2) production. These observations indicate that catechins increase the prostacyclin production and that the pyrogallol structure is significant to this function.
我们描述了茶中已知的儿茶素之一（-）表没食子儿茶素没食子酸酯（EGCg）对牛主动脉内皮细胞前列环素（PGI）生成的影响。使用气相色谱/选择离子监测（GC/SIM）测定培养基中释放的PGI（2）和PGI（3）的稳定代谢产物6-酮-PGF（1α）和Δ（17）-6-酮-PGF（1α）的量。EGCg以剂量和时间依赖性方式增加内皮细胞的前列环素生成。EGCg的作用比任何其他儿茶素（儿茶素、表儿茶素、表没食子儿茶素和表儿茶素没食子酸酯）都更强。当内皮细胞与EGCg和花生四烯酸（AA）或二十碳五烯酸（EPA）一起孵育时，PGI（2）和PGI（3）的生成比单独与AA或EPA孵育时增加得更多。此外，同样具有邻苯三酚结构的没食子酸增加了PGI（2）的生成。这些观察结果表明，儿茶素增加前列环素的生成，并且邻苯三酚结构对该功能具有重要意义。
