铅作业工人δ-氨基乙酰丙酸脱水酶基因型与血红素前体之间的关系
Relationship between delta-aminolevulinic acid dehydratase genotypes and heme precursors in lead workers.
作者信息
Sakai T, Morita Y, Araki T, Kano M, Yoshida T
机构信息
Center of Occupational Medicine, Tokyo Rosai Hospital, Tokyo, Japan.
出版信息
Am J Ind Med. 2000 Sep;38(3):355-60. doi: 10.1002/1097-0274(200009)38:3<355::aid-ajim17>3.0.co;2-6.
BACKGROUND
The objective of this study was to investigate the relationships between genotypes of delta-aminolevulinic acid (ALA) dehydratase (ALAD) and disturbances in the heme biosynthetic pathway by lead exposure.
METHODS
The subjects were 192 male lead workers and 125 control subjects. Blood lead concentrations (Pb-B), plasma ALA concentrations (ALA-P), and ALAD genotypes were determined for all subjects. In lead workers, ALAD activity, ALA in urine (ALA-U), and erythrocyte zinc protoporphyrin (ZP) were also determined.
RESULTS
The frequency of ALAD2 (minor type of ALAD allele) was calculated to be 0.087 in all subjects. No significant relationship was found between ALAD2 frequency and Pb-B levels in lead workers. ALAD1 homozygotes showed significantly higher levels of ZP and ALA-P in comparison with those of ALAD2 carriers at Pb-B levels more than 20 microg/dL and 40 microg/dL, respectively.
CONCLUSIONS
ALAD1 homozygotes might be more susceptible than ALAD2 carriers to disturbances in heme metabolism caused by lead exposure.
背景
本研究的目的是调查δ-氨基乙酰丙酸（ALA）脱水酶（ALAD）的基因型与铅暴露导致的血红素生物合成途径紊乱之间的关系。
方法
研究对象为192名男性铅作业工人和125名对照者。测定了所有研究对象的血铅浓度（Pb-B）、血浆ALA浓度（ALA-P）以及ALAD基因型。对于铅作业工人，还测定了ALAD活性、尿ALA（ALA-U）和红细胞锌原卟啉（ZP）。
结果
所有研究对象中ALAD2（ALAD等位基因的次要类型）的频率经计算为0.087。在铅作业工人中，未发现ALAD2频率与Pb-B水平之间存在显著关系。在Pb-B水平分别超过20μg/dL和40μg/dL时，与ALAD2携带者相比，ALAD1纯合子的ZP和ALA-P水平显著更高。
结论
与ALAD2携带者相比，ALAD1纯合子可能对铅暴露引起的血红素代谢紊乱更敏感。
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