Sigala S, Missale C, Tognazzi N, Spano P
Department of Biomedical Sciences and Biotechnologies, Brescia, University Medical School, Italy.
Neuroreport. 2000 Aug 3;11(11):2467-71. doi: 10.1097/00001756-200008030-00025.
Chromaffin cells and sympathetic neurons arise from a common bipotential progenitor which, if exposed to nerve growth factor (NGF), matures into a sympathetic neuron, but if exposed to glucocorticoids (GCs), differentiates into a mature chromaffin cell. Pharmacological evidence indicates that, in adrenal medulla and sympathetic neurons, dopamine (DA) receptors belonging to the D-2 family inhibit catecholamine secretion. The molecular characterization of these receptors, however, is not been yet described. Our data suggest that bipotential cells obtained from newborn rat adrenal medulla express both isoforms of the D-2 receptor, while D-3 receptor and D-4 receptor messenger RNAs (mRNAs) are not present. GC-mediated maturation induces the expression of D-4 receptors, without modification of D-2 isoforms. Sympathetic neurons differentiated in vitro selectively express the D-2short mRNA. Taken together, present results suggest that NGF and GCs play a role in regulating D-2 family receptor expression in neural crest-derived cells.
嗜铬细胞和交感神经元起源于一种共同的双潜能祖细胞，该祖细胞如果暴露于神经生长因子（NGF），则会成熟为交感神经元，但如果暴露于糖皮质激素（GCs），则会分化为成熟的嗜铬细胞。药理学证据表明，在肾上腺髓质和交感神经元中，属于D-2家族的多巴胺（DA）受体抑制儿茶酚胺分泌。然而，这些受体的分子特征尚未被描述。我们的数据表明，从新生大鼠肾上腺髓质获得的双潜能细胞表达D-2受体的两种亚型，而不存在D-3受体和D-4受体信使核糖核酸（mRNAs）。GC介导的成熟诱导D-4受体的表达，而D-2亚型没有改变。体外分化的交感神经元选择性地表达D-2短mRNA。综上所述，目前的结果表明，NGF和GCs在调节神经嵴衍生细胞中D-2家族受体表达方面发挥作用。
