Chattopadhyay D, Smith C D, Barchue J, Langsley G
Division of Geographic Medicine, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Acta Crystallogr D Biol Crystallogr. 2000 Aug;56(Pt 8):1017-9. doi: 10.1107/s0907444900006417.
The Plasmodium falciparum rab6 gene encodes a 208 amino-acid polypeptide. Two recombinant versions of P. falciparum Rab6 protein were expressed in Escherichia coli: the full-length protein and a truncated form containing residues 1-175. Both forms were purified from the soluble fraction of bacterial extract and were purified by ion-exchange chromatography and size-exclusion chromatography. Purified proteins were crystallized at pH 6.5 using the hanging-drop vapor-diffusion technique at room temperature. The full-length protein diffracted to 2.4 A and belongs to the tetragonal space group P4(3)2(1)2 or P4(1)2(1)2, with unit-cell parameters a = b = 80. 6, c = 90.4 A. The crystals of the truncated protein were isomorphous with those of the full-length construct and diffracted X-rays to 2.2 A resolution.
恶性疟原虫rab6基因编码一种208个氨基酸的多肽。恶性疟原虫Rab6蛋白的两种重组形式在大肠杆菌中表达：全长蛋白和包含1 - 175位残基的截短形式。两种形式均从细菌提取物的可溶部分纯化，并通过离子交换色谱和尺寸排阻色谱进行纯化。使用悬滴气相扩散技术在室温下于pH 6.5条件下将纯化的蛋白质结晶。全长蛋白衍射至2.4 Å，属于四方晶系空间群P4(3)2(1)2或P4(1)2(1)2，晶胞参数a = b = 80.6，c = 90.4 Å。截短蛋白的晶体与全长构建体的晶体同晶型，X射线衍射分辨率为2.2 Å。
