酿酒酵母过氧化物酶体Δ3-Δ2-烯酰辅酶A异构酶的结晶及X射线衍射分析

Crystallization and X-ray diffraction analysis of peroxisomal Delta3-Delta2-enoyl-CoA isomerase from Saccharomyces cerevisiae.

作者信息

Mursula A M, van Aalten D M, Modis Y, Hiltunen J K, Wierenga R K

机构信息

Biocenter Oulu and Department of Biochemistry, University of Oulu, BOX 3000, FIN-90014 Oulun Yliopisto, Finland.

出版信息

Acta Crystallogr D Biol Crystallogr. 2000 Aug;56(Pt 8):1020-3. doi: 10.1107/s0907444900006533.

DOI:10.1107/s0907444900006533

PMID:10944342

Abstract

The purification, crystallization and X-ray diffraction analysis of Saccharomyces cerevisiae Delta(3)-Delta(2)-enoyl-CoA isomerase is described. Delta(3)-Delta(2)-Enoyl-CoA isomerase is a member of the hydratase/isomerase protein family and is an auxiliary enzyme required for the beta-oxidation of unsaturated fatty acids. It is a hexameric enzyme consisting of six identical 32 kDa subunits of 280 residues each. In crystallization trials three crystal forms were obtained, with tetragonal and hexagonal lattices. A 2.5 A data set was collected from the unliganded hexagonal crystals with an R(merge) of 6.6%. The crystal, with unit-cell parameters a = 116.0, b = 116.0, c = 122.9 A, is likely to have P6(3)22 symmetry.

摘要

本文描述了酿酒酵母Δ(3)-Δ(2)-烯酰辅酶A异构酶的纯化、结晶及X射线衍射分析。Δ(3)-Δ(2)-烯酰辅酶A异构酶是水合酶/异构酶蛋白家族的成员，是不饱和脂肪酸β氧化所需的辅助酶。它是一种六聚体酶，由六个相同的32 kDa亚基组成，每个亚基有280个残基。在结晶试验中，获得了三种晶体形式，具有四方和六方晶格。从无配体的六方晶体收集了一个2.5 Å的数据集，合并R值为6.6%。该晶体的晶胞参数为a = 116.0、b = 116.0、c = 122.9 Å，可能具有P6(3)22对称性。