Sanger J W
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2451-5. doi: 10.1073/pnas.72.6.2451.
Actin has been shown to be present in the nucleoli, kinetochore and centriolar regions, and in the mitotic spindle of rat kangaroo cells which have been stained with fluorescently labeled heavy meromyosin. The actin in the spindle is confined to the fibers that connect the chromosomes with the centriolar region. Actin was not present in astral fibers, in the continuous spindle fibers that connect the poles, or in non-kinetochore regions of the chromosomes. The specific localization of actin in chromosomal spindle fibers suggests an actin-mvosin interaction as the force-producing mechanism for chromosomal movement.
已证明，在用荧光标记的重酶解肌球蛋白染色的大鼠袋鼠细胞中，肌动蛋白存在于核仁、动粒和中心粒区域以及有丝分裂纺锤体中。纺锤体中的肌动蛋白局限于将染色体与中心粒区域相连的纤维中。星射线、连接两极的连续纺锤体纤维或染色体的非动粒区域中均不存在肌动蛋白。肌动蛋白在染色体纺锤体纤维中的特定定位表明，肌动蛋白与肌球蛋白的相互作用是染色体运动的力产生机制。
