Kanemitsu Y
Department of Psychosomatic Medicine, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan.
Fukuoka Igaku Zasshi. 2000 Jul;91(7):170-83.
Changes in the expressions of interleukin-1 beta (IL-1 beta) mRNA in the rat brain were investigated during inflammatory or non-inflammatory stress. We utilized competitive reverse transcription PCR (RT-PCR) to quantitate precisely the minute amount of IL-1 beta mRNA in small tissues which were micro-punched out from discrete sites in the brain. Intraperitoneal injection of lipopolysaccharide (100 micrograms/kg) caused a widespread and high elevation of IL-1 beta mRNA in the hypothalamus, hippocampus and cerebral cortex. Immobilization stress significantly increased IL-1 beta mRNA only in the hypothalamus, when examined in the brain block samples, which reached a peak 30 minutes after the start of stress. However, examination of the small tissues punched out from discrete sites in the brain revealed a profound elevation of IL-1 beta mRNA in the CA1 region of the hippocampus and in all the hypothalamic nuclei examined 30 minutes after the start of immobilization stress, but not in the CA3 region and the dentate gyrus or cerebral cortex. The method shown in this report proved to be useful and may be applied to quantify the low amount of mRNA in the small areas in the brain.
我们研究了在炎症或非炎症应激期间大鼠脑中白细胞介素-1β（IL-1β）mRNA表达的变化。我们利用竞争性逆转录PCR（RT-PCR）精确量化从小脑离散部位微穿孔取出的小组织中微量的IL-1βmRNA。腹腔注射脂多糖（100微克/千克）导致下丘脑、海马体和大脑皮层中IL-1βmRNA广泛且高度升高。当在脑块样本中检测时，固定应激仅在下丘脑中显著增加IL-1βmRNA，在应激开始后30分钟达到峰值。然而，对从小脑离散部位穿孔取出的小组织进行检测发现，固定应激开始后30分钟，海马体CA1区和所有检测的下丘脑核中IL-1βmRNA显著升高，但在CA3区、齿状回或大脑皮层中未升高。本报告中所示方法证明是有用的，可用于量化脑中小区域内的低水平mRNA。
