Nagafuchi S, Totsuka M, Hachimura S, Goto M, Takahashi T, Yajima T, Kuwata T, Kaminogawa S
Nutrition Science Institute, Meiji Milk Products Co., Ltd., Odawara, Kanagawa, Japan.
Biosci Biotechnol Biochem. 2000 Jul;64(7):1459-65. doi: 10.1271/bbb.64.1459.
We have investigated the effects of dietary nucleotides on intraepithelial lymphocytes (IEL) and intestinal epithelial cells (IEC) in weanling mice. The proportion of T-cell receptor (TCR) gammadelta+ IEL in BALB/c mice fed a diet supplemented with nucleotides (NT(+) diet) was significantly higher than that in mice fed the nucleotide-free diet, while the proportion of TCR alphabeta+ IEL in NT(+) diet-fed mice was significantly decreased. The change of the TCR alphabeta+/TCR gammadelta+ ratio was mainly observed in a CD8 alphaalpha+ subset of IEL. IEC from NT(+) diet-fed mice produced a higher level of IL-7, which is important in the development of TCR gammadelta+ IEL, than those from control diet-fed mice. The expression levels of IL-7 and IL-2 receptors on IEL were not different between the two dietary groups. Our findings suggest that the increased population of a TCR gammadelta+ IEL subset by feeding nucleotides may be caused by the increased production of IL-7 by IEC.
我们研究了日粮核苷酸对断奶小鼠上皮内淋巴细胞（IEL）和肠上皮细胞（IEC）的影响。喂食添加核苷酸日粮（NT(+)日粮）的BALB/c小鼠中，T细胞受体（TCR）γδ+ IEL的比例显著高于喂食无核苷酸日粮的小鼠，而喂食NT(+)日粮的小鼠中TCRαβ+ IEL的比例显著降低。TCRαβ+/TCRγδ+ 比值的变化主要在IEL的CD8αα+亚群中观察到。与喂食对照日粮的小鼠相比，喂食NT(+)日粮的小鼠的IEC产生更高水平的IL-7，IL-7在TCRγδ+ IEL的发育中起重要作用。两个日粮组之间IEL上IL-7和IL-2受体的表达水平没有差异。我们的研究结果表明，通过喂食核苷酸增加TCRγδ+ IEL亚群的数量可能是由于IEC产生的IL-7增加所致。
