Aoki T, Miyashita M, Fujino H, Watabe H
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan.
Biosci Biotechnol Biochem. 2000 Jul;64(7):1547-51. doi: 10.1271/bbb.64.1547.
A green fluorescent protein mutant (S147P GFP) was fused with protein A and expressed in Escherichia coli. This fusion protein (PA-GFP147) was used in immunoblotting studies as a new detection system, designated as "flexible single-step detection (FSSD)". In FSSD, the detection of blotted antigen was done in one step, and the antigen-antibody reaction can be monitored by UV-irradiation in real time. The reaction time, therefore, can be flexibly controlled by monitoring the green fluorescence.
一种绿色荧光蛋白突变体（S147P GFP）与蛋白A融合，并在大肠杆菌中表达。这种融合蛋白（PA-GFP147）被用作免疫印迹研究中的一种新检测系统，命名为“灵活单步检测（FSSD）”。在FSSD中，印迹抗原的检测一步完成，抗原-抗体反应可通过紫外线照射实时监测。因此，通过监测绿色荧光可灵活控制反应时间。
